DNA barcoding Australia's fish species.
نویسندگان
چکیده
Two hundred and seven species of fish, mostly Australian marine fish, were sequenced (barcoded) for a 655 bp region of the mitochondrial cytochrome oxidase subunit I gene (cox1). Most species were represented by multiple specimens, and 754 sequences were generated. The GC content of the 143 species of teleosts was higher than the 61 species of sharks and rays (47.1% versus 42.2%), largely due to a higher GC content of codon position 3 in the former (41.1% versus 29.9%). Rays had higher GC than sharks (44.7% versus 41.0%), again largely due to higher GC in the 3rd codon position in the former (36.3% versus 26.8%). Average within-species, genus, family, order and class Kimura two parameter (K2P) distances were 0.39%, 9.93%, 15.46%, 22.18% and 23.27%, respectively. All species could be differentiated by their cox1 sequence, although single individuals of each of two species had haplotypes characteristic of a congener. Although DNA barcoding aims to develop species identification systems, some phylogenetic signal was apparent in the data. In the neighbour-joining tree for all 754 sequences, four major clusters were apparent: chimaerids, rays, sharks and teleosts. Species within genera invariably clustered, and generally so did genera within families. Three taxonomic groups-dogfishes of the genus Squalus, flatheads of the family Platycephalidae, and tunas of the genus Thunnus-were examined more closely. The clades revealed after bootstrapping generally corresponded well with expectations. Individuals from operational taxonomic units designated as Squalus species B through F formed individual clades, supporting morphological evidence for each of these being separate species. We conclude that cox1 sequencing, or 'barcoding', can be used to identify fish species.
منابع مشابه
Species identification reveals mislabeling of important fish products in Iran by DNA barcoding
This study reports on the molecular identification of fish species from processed products which had a priori been classified as belonging to 5 important species in Iran for human consumption. DNA barcoding using direct sequencing of an approximately 650bp of mitochondrial Cytochrome oxidase subunit I (COI) gene revealed incorrect labeling of Narrow-barred Spanish mackerel samples. High occurre...
متن کاملSpecies identification reveals mislabeling of important fish products in Iran by DNA barcoding
This study reports on the molecular identification of fish species from processed products which had a priori been classified as belonging to 5 important species in Iran for human consumption. DNA barcoding using direct sequencing of an approximately 650bp of mitochondrial Cytochrome oxidase subunit I (COI) gene revealed incorrect labeling of Narrow-barred Spanish mackerel samples. High occurre...
متن کاملResearch Article: Molecular genetic divergence of five genera of cypriniform fish in Iran assessed by DNA barcoding
The present study represents a comprehensive molecular assessment of some family of freshwater fishes in Iran. We analyzed cytochrome oxidase I (COI) sequences for five genus of cypriniform fishes from Iran. The present investigation provides data on genetic structure of some species of Nemachilidae including Paraschistura bampurensis, Oxynoemacheilus kiabii and Turcinemacheilus saadii and Leuc...
متن کاملA DNA Mini-Barcoding System for Authentication of Processed Fish Products
Species substitution is a form of seafood fraud for the purpose of economic gain. DNA barcoding utilizes species-specific DNA sequence information for specimen identification. Previous work has established the usability of short DNA sequences-mini-barcodes-for identification of specimens harboring degraded DNA. This study aims at establishing a DNA mini-barcoding system for all fish species com...
متن کاملDNA barcodes discriminate freshwater fishes from the Paraíba do Sul River Basin, São Paulo, Brazil.
BACKGROUND AND AIMS Considering the promising use of DNA barcoding for species identification, the importance of the freshwater fish fauna of the Paraíba do Sul River Basin, and its advanced stage of degradation, the present study evaluated the effectiveness of DNA barcoding to identify the fish species in this basin. MATERIALS AND METHODS A total of 295 specimens representing 58 species belo...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Philosophical transactions of the Royal Society of London. Series B, Biological sciences
دوره 360 1462 شماره
صفحات -
تاریخ انتشار 2005