An unexpected signature of Lorenz-Mie scattering observed in FlowCytometric experiments
نویسندگان
چکیده
Detailed analysis of elastic light scattering by spheres in FlowCytometers shows unexpected Lorenz-Mie scattering patterns. The complete scattering matrix S of spheres was measured. Two parameter scatterplots with xand ycoordinates determined by the S11 + Sij and S11 Sij values were obtained. Samples of spheres with very narrow size distributions were analyzed and produced unexpected two parameter scatterplots. Instead of compact distributions we observed Lissajous-like loops. Simulation of the scatterplots, using Lorenz-Mie theory, shows that these loops are not due to experimental errors, but due to true Lorenz-Mie scattering. We show that the loops originate from the sensitivity of the scattered field on the radius of the spheres. This work demonstrates that the interpretation of rare events and hidden features in FlowCytometry needs reconsideration.
منابع مشابه
Another face of Lorenz-Mie scattering: monodisperse distributions of spheres produce Lissajous-like patterns.
The complete scattering matrix S of spheres was measured with a flow cytometer. The experimental equipment allows simultaneous detection of two scattering-matrix elements for every sphere in the distribution. Two-parameter scatterplots with x and y coordinates determined by the S(ll) + S(ij) and S(ll)-S(ij) values are measured. Samples of spheres with very narrow size distributions (< 1%) were ...
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