Screening of Blood-brain Barrier Penetration Using the Immobilized Artificial Membrane Phosphatidylcholine Column Chromatography at the Physiological Ph

In this study, 21 structurally diverse drugs with examined central nervous system penetration were used for prediction of blood-brain barrier penetration using HPLC-UV instrument. Drugs' chromatographic capacity factors were measured by the immobilized artificial membrane presented by phosphatidylcholine column. The correlation between chromatographic capacity factor, octanol-water partition coefficient (log P) and molecular polar surface area (PSA) were determined at the physiological pH. The correlation factor 0.6677 with respect to log P and 0.7199 in reference to PSA was assigned. The developed in vitro prediction method may be used as a screening tool for blood-brain barrier penetration of drugs with passive transport mechanism.