Glyoxalase I in micro-organisms: molecular characteristics, genetics and biochemical regulation.

Introduction Recombinant DNA techniques have revolutionized the field of microbiology. The techniques are quite helpful to analyse and modify various functions of cells. Using recombinant DNA techniques we have succeeded in analysing and modifying the metabolic functions of micro-organisms [ l]. Focusing on the glyoxalase system we have analysed a glycolytic-methylglyoxal cycle and have produced a ‘jumbo’ yeast, which could produce a large amount of S-lactoylglutathione [2-41.