Targeting essential genes in Salmonella enterica serovar typhimurium with antisense peptide nucleic acid.
نویسندگان
چکیده
We investigated the capability of antisense peptide nucleic acids (PNAs) conjugated to the (KFF)(3)K cell-penetrating peptide to target possible essential genes (ligA, rpoA, rpoD, engA, tsf, and kdtA) in Salmonella enterica serovar Typhimurium and inhibit bacterial growth in vitro and in cell culture. All targeted PNA-based gene inhibition has shown great potency in gene expression inhibition in a sequence-specific and dose-dependent manner at micromolar concentrations. Among tested PNAs, the anti-rpoA and -rpoD PNAs showed the greatest potency.
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 56 12 شماره
صفحات -
تاریخ انتشار 2012