Autophagy alleviates LPS induced Hyper-Permeability of Caco-2 cells by regulating claudin-2 protein
نویسندگان
چکیده
To investigate the effect of autophagy on claudin-2 in lipopolysaccharide (LPS) induced hyper-permeability of intestinal epithelial cell. In human colon cancer cell line (Caco-2), the model of intestinal epithelial injury induced by LPS was established, which were LPS group, LPS combined with inhibitor of autophagy (3-Methyladenine, 3-MA) and control group. Transepithelial resistance (TER) was detected by Millicell-ERS and monolayer permeability was evaluated by paracellular transport of fluorescein isothiocyanate dextran (FITC-dextran).Expression of LC3II and claudins-2 in Caco-2 cells were analyzed by Western blot. Expression of mRNA of CL-2 was detected by RT-PCR. The results showed that LPSinduced the expression of LC3II was increased, and TER was decreased compared with control group. Moreover, claudin-2 and monolayer permeability were up-regulated in LPS + 3-MA group compared with LPS group, and TER was significantly decreased compared with LPS group and control group. Autophagy may down regulated the expression of CL-2 in intestinal epithelial cells induced by LPS, and reduce intestinal epithelial permeability to protect intestinal mucosal barrier.
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