Specific Gene bciD for C7-Methyl Oxidation in Bacteriochlorophyll e Biosynthesis of Brown-Colored Green Sulfur Bacteria

نویسندگان

  • Jiro Harada
  • Tadashi Mizoguchi
  • Souichirou Satoh
  • Yusuke Tsukatani
  • Makio Yokono
  • Masato Noguchi
  • Ayumi Tanaka
  • Hitoshi Tamiaki
چکیده

The gene named bciD, which encodes the enzyme involved in C7-formylation in bacteriochlorophyll e biosynthesis, was found and investigated by insertional inactivation in the brown-colored green sulfur bacterium Chlorobaculum limnaeum (previously called Chlorobium phaeobacteroides). The bciD mutant cells were green in color, and accumulated bacteriochlorophyll c homologs bearing the 7-methyl group, compared to C7-formylated BChl e homologs in the wild type. BChl-c homolog compositions in the mutant were further different from those in Chlorobaculum tepidum which originally produced BChl c: (3(1) S)-8-isobutyl-12-ethyl-BChl c was unusually predominant.

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Complete Genome Sequence of the Photoautotrophic and Bacteriochlorophyll e-Synthesizing Green Sulfur Bacterium Chlorobaculum limnaeum DSM 1677T

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عنوان ژورنال:

دوره 8  شماره 

صفحات  -

تاریخ انتشار 2013