HEMATOPOIESIS AND STEM CELLS Mef2C is a lineage-restricted target of Scl/Tal1 and regulates megakaryopoiesis and B-cell homeostasis

نویسندگان

  • Christos Gekas
  • Katrin E. Rhodes
  • Laurraine M. Gereige
  • Hildur Helgadottir
  • Roberto Ferrari
  • Siavash K. Kurdistani
  • Encarnación Montecino-Rodriguez
  • Rhonda Bassel-Duby
  • Eric Olson
  • Andrei V. Krivtsov
  • Scott Armstrong
  • Stuart H. Orkin
  • Matteo Pellegrini
  • Hanna K. A. Mikkola
چکیده

1Department of Molecular, Cell and Developmental Biology, University of California–Los Angeles; 2Department of Pediatric Oncology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA; 3Department of Biologic Chemistry, University of California–Los Angeles; 4Department of Pathology and Laboratory Medicine, University of California–Los Angeles; 5Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas; 6Division of Hematology/Oncology, Children’s Hospital Boston, Harvard Medical School, MA; 7Howard Hughes Medical Institute, Boston, MA; 8Harvard Stem Cell Institute, Boston, MA; 9Molecular Biology Institute, University of California–Los Angeles; 10Eli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at University of California–Los Angeles; and 11Jonsson Comprehensive Cancer Center, University of California–Los Angeles

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Mef2C is a lineage-restricted target of Scl/Tal1 and regulates megakaryopoiesis and B-cell homeostasis.

The basic helix-loop-helix transcription factor stem cell leukemia gene (Scl) is a master regulator for hematopoiesis essential for hematopoietic specification and proper differentiation of the erythroid and megakaryocyte lineages. However, the critical downstream targets of Scl remain undefined. Here, we identified a novel Scl target gene, transcription factor myocyte enhancer factor 2 C (Mef2...

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Low SCL/TAL1 expression reveals its major role in adult hematopoietic myeloid progenitors and stem cells.

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Tetracycline-controlled transgenic targeting from the SCL locus directs conditional expression to erythrocytes, megakaryocytes, granulocytes, and c-kit-expressing lineage-negative hematopoietic cells.

The stem cell leukemia gene SCL, also known as TAL-1, encodes a basic helix-loop-helix transcription factor expressed in erythroid, myeloid, megakaryocytic, and hematopoietic stem cells. To be able to make use of the unique tissue-restricted and spatio-temporal expression pattern of the SCL gene, we have generated a knock-in mouse line containing the tTA-2S tetracycline transactivator under the...

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SCL/TAL1 expression level regulates human hematopoietic stem cell self-renewal and engraftment.

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MEIS1 Regulates Hemogenic Endothelial Generation, Megakaryopoiesis, and Thrombopoiesis in Human Pluripotent Stem Cells by Targeting TAL1 and FLI1

Human pluripotent stem cells (hPSCs) provide an unlimited source for generating various kinds of functional blood cells. However, efficient strategies for generating large-scale functional blood cells from hPSCs are still lacking, and the mechanism underlying human hematopoiesis remains largely unknown. In this study, we identified myeloid ectopic viral integration site 1 homolog (MEIS1) as a c...

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تاریخ انتشار 2009