Antibody formation for malignant tumor. II. Antigenicity of Ehrlich ascites tumor lipoprotein.

A unique low density lipoprotein was obtained from the tumor transplanted with a cultured cell line of Ehrlich ascites tumor, JTC-ll cell. The tumor low density lipoprotein electrophoretically migrated as a single band, and the mobility was different from that of other organs. The chemical composition of lipid, cholesterol and phospholipids in tumor low density lipoprotein were characteristic. The flotation rate was Sf 5.9, and thus the molecular weight was estimated to be about 130 x 104. The inhibitory effect on tumor growth of the immune serum was most elevated at 25th day after the intraperitoneal administration of tumor low density lipoprotein. The main fraction effective for inhibition of tumor growth existed in γ-globulin. ∗PMID: 4256471 [PubMed indexed for MEDLINE] Copyright c ©OKAYAMA UNIVERSITY MEDICAL SCHOOL Acta Merl. Okayama 24, 527-536 (1970) ANTIBODY FORMATION FOR MALIGNANT TUMOR II. ANTIGENICITY OF EHRLICH ASCITES TUMOR LIPOPROTEIN Yasuhisa YAMAMOTO, Yasuo TADATOMO and Shinsuke OKAMURA Department oj Surgery, Okayama University Medical School, Okayama, Japan (Director: Prof. S. Tanaka) Received Jar publication, July 21, 1970 Tumor specific antigenicity has been clarified from the aspect of genetical compatible host. It has been discovered by FOLEY (l) and has been confirmed by PREHN (2), and KLEIN (3) that methylcholanthreneinduced sarcomas in mice are strongly antigenic. They reported that the antigenicity was tumor specific and that it did not depend on genetically determined isoantigenic differences between donor and recipient. In the previous report (4), the antitumor activity of regional lymph node protein of mice transplanted with a cultured cell line of Ehrlich ascites tumor, ]TC-II cell, was demonstrated. In the present experiment, the low density lipoprotein in tumor of dd mice transplanted with ]TC-II cells was prepared by dextran sulfate precipitation method. Tumor low density lipoprotein showed a single band in electrophoresis, and the chemical characters were different from those of other organs. The growth inhibitory effect was observed in vitro cultured JTC-II cells by the serum immunized with the tumor low density Iipoprotein.