1ninnino logical Detection of Aflatoxin Bi-DNA Adducts Formed in y ivo1
نویسندگان
چکیده
Two monoclonal antibodies (6A10 and 12F5) were obtained after fusion of mouse P3X63-AG.8.653 myeloma cells with spleen cells isolated from BALB/c mice immunized with imidazole ring-opened aflatoxin H, (AI If, )DNA and characterized by competitive enzyme-linked immunosorbent assays. Both antibodies are highly specific for imidazole ring opened AFBi-DNA and show some cross-reactivity with AI H,-UNA and no cross-reactivity with 8,9-dihydro-8-(7-guanyl)-9-hydroxy-AFBi, AFBi conjugated with bovine serum albumin, aflatoxin M, conjugated with bovine serum albumin, AI It,, or aflatoxin d. Antibody 6A10 was further characterized and showed no cross-reactivity with DNA modified by several other carcinogens. It could detect adducts with 4-fold higher sensitivity in highly modified DNA (2.5 adducts/100 nucleotides) than in low modified DNA (4 adducts/10s nucleotides). With low modified DNA the limit of sensitivity is 5 adducts/10T nucleotides. Antibody 6A10 reliably detected adducts formed in vivo in rats and mice treated with AFBi. In a pilot study, UH, adducts were detected in liver tissues from individuals living in areas with suspected exposure to AFBi. Monitoring adduct levels in human tissue may provide information not only on carcinogen exposure but also on the relationship among infection with hepatitis B virus, dietary exposure to aflatoxin It,, and liver cancer.
منابع مشابه
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