Expression of muscle-gene-specific isozymes of phosphorylase and creatine kinase in innervated cultured human muscle
نویسندگان
چکیده
Isozymes of creatine kinase and glycogen phosphorylase are excellent markers of skeletal muscle maturation. In adult innervated muscle only the muscle-gene-specific isozymes are present, whereas aneurally cultured human muscle has predominantly the fetal pattern of isozymes. We have studied the isozyme pattern of human muscle cultured in monolayer and innervated by rat embryo spinal cord explants for 20-42 d. In this culture system, large groups of innervated muscle fibers close to the ventral part of the spinal cord explant continuously contracted. The contractions were reversibly blocked by 1 mM d-tubocurarine. In those innervated fibers, the total activity and the muscle-gene-specific isozymes of both enzymes increased significantly. The amount of muscle-gene-specific isozymes directly correlated with the duration of innervation. Control noninnervated muscle fibers from the same dishes as the innervated fibers remained biochemically immature. This study demonstrated that de novo innervation of human muscle cultured in monolayer exerts a time-related maturational influence that is not mediated by a diffusable neural factor.
منابع مشابه
Electrophysiologic and clinico-pathologic characteristics of statin-induced muscle injury
Objective(s):In this study, we aimed at evaluation of electrophysiological and histopathalogical characteristics of statin-induced muscle injury as well as clinical features of patients who develop this condition in terms of frequency and pattern of evolution. Materials and Methods: Forty patients (age 39-74 years) including 25 subjects with type 2 diabetes mellitus, 9 with cardiovascular disea...
متن کاملThe Effect of Eight Weeks Aerobic and Resistance Training on AMP-Activated Protein Kinase (AMPK) Gene Expression in Soleus Muscle and Insulin Resistance of STZ-Induced Diabetic Rat
Background: AMPK regulation is one of biggest target in T2D and metabolic syndrome research. Therefore, the present study is aimed to investigate The effect of 8 weeks aerobic and Resistance training on AMP-activated protein kinase (AMPK) gene expression in soleus muscle and insulin resistance of STZ-induced diabetic rat. Methods: The research method of present study was experimental. For this...
متن کاملA comparative study of human muscle and brain creatine kinases expressed in Escherichia coli.
We report the expression of the human muscle (CK-MM) and brain (CK-BB) creatine kinases in Escherichia coli. The proteins have been purified to apparent homogeneity and several of their physical and kinetic properties investigated. In the process, we have conclusively verified the correct DNA sequence of the genes encoding the respective isozymes, and determined the correct primary structure an...
متن کاملExpression of muscle-type phosphorylase in innervated and aneural cultured muscle of patients with myophosphorylase deficiency.
Patients with McArdle's myopathy lack muscle glycogen phosphorylase (M-GP) activity. Regenerating and cultured muscle of patients with McArdle's myopathy presents a glycogen phosphorylase (GP) activity, but it is not firmly established whether M-GP or non-M-GP isoforms are expressed. We have cultured myoblasts from biopsy specimen of five patients with McArdle's myopathy. Skeletal muscle was cu...
متن کاملThe Effect of Adiponectin on Matrix Metalloproteinase-9 (MMP-9) in Vascular Smooth Muscle Cells
Background & Aims: Atherosclerosis is a major cause of morbidity and mortality. Adiponectin reducesthe risk of heart disease, and matrix metalloproteinase-9 (MMP-9) is involved in the formation and development of atherosclerotic plaque. The aim of this study was the investigation of the effect of adiponectin on MMP-9 gene expression. It seems this hormone can reduce the risk of atherosclerosis ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Journal of Cell Biology
دوره 103 شماره
صفحات -
تاریخ انتشار 1986