Butyl rubber stoppers increase the shelf life of prereduced, anaerobically sterilized media.
نویسندگان
چکیده
Butyl rubber stoppers as compared with neoprene or black rubber stoppers significantly increased the shelf life of prereduced, anaerobically sterilized media for growth of obligately anaerobic bacteria.
منابع مشابه
Sterilization of rotary NiTi instruments within endodontic sponges.
AIM To determine whether the following can be sterilized by autoclaving - endodontic sponges, rotary nickel-titanium (NiTi) instruments within endodontic sponges, and rotary NiTi instruments with rubber stoppers. METHODOLOGY Sixty-four samples of eight different endodontic sponges (n = 512) were placed into brain heart infusion broth (BHI) for 72 h. An aliquot of this was then spread onto hor...
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Both prereduced molten agar and broth and aerobic molten agar and broth were inoculated with blood samples collected from patients with periodontitis, but in otherwise good health, both before and after extraction of two or more teeth. Postoperative blood samples from 23 of 25 patients sampled yielded anaerobic and facultative species. Colony counts from nine samples yielded from less than 1 to...
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Stringently anaerobic bacteria have been successfully cultured in rubber-stoppered roll tubes. Stoppers of certain types of rubber are so permeable to oxygen that they are unsuitable (Hungate, J. Bacteriol. 86:848, 1963). Butyl rubber is less permeable to gases than are many other types. The permeabilities of butyl stoppers and a common laboratory type to gases concerned in anaerobic culture wo...
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ورودعنوان ژورنال:
- Applied and environmental microbiology
دوره 33 5 شماره
صفحات -
تاریخ انتشار 1977