Endo-β-mannanase from the endosperm of seeds of Sesbania virgata (Cav.) Pers. (Leguminosae): purification, characterisation and its dual role in germination and early seedling growth
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چکیده
Abbreviations: ABA, abscisic acid; BSA, bovine serum albumin; CAP, micropylar area around the radicle emergence point or endosperm cap; DAB, diaminobenzidine; DEAE, diethylaminoethyl; IgG, immunoglobulin G; LAT, lateral coat plus endosperm; LBG, locust bean gum; ηsp, specific viscosity; PBS, phosphate buffer saline; pI, isoelectric point; RAD, radicle tip; SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis; Visc. U., viscometric units.
منابع مشابه
A novel antifungal protein from seeds of Sesbania virgata (Cav.) Pers. (Leguminosae-Faboideae).
A novel antifungal protein with a molecular mass around 50 kDa was purified from seeds of Sesbania virgata (Cav.) Pers. using ammonium sulfate fractionation followed by gel filtration on a Sephadex G-75 Superfine (Sigma) column and reverse-phase high performance liquid chromatography on a C8 column. The protein, designated FP1-A, with a novel N-terminal sequence AMVHSPGG(S)FS(P), showed growth ...
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