Experimental Studies on the Sexual Cycle of the South African Clawed Toad (xenopus

Cell-free extracts of eggs of the South African clawed toad Xenopus faevis

Cell-free extracts of eggs of the South African clawed toad Xenopus faevis support complete chromosome replication under normal cell cycle control (Blow and Laskey 1986). Apart from a similar system derived from embryos of the fruit fly Drosophifa mefanogaster (Crevel and Cotterill 1991), it is currently the only eukaryotic cell-free system that supports efficient chromosome replication in vitr...

The Incidence of Pathologic Abnormalities, including Spontaneous Lymphosarcomas, in a Laboratory Stock of Xenopus (the South African Clawed Toad).

Amphibian hormonal induction

Conservation breeding programs for the flagship species, the Wyoming toad (Bufo baxteri), Boreal toad (Bufo boreas boreas), Puerto Rican crested toad (Peltophryne lemur), and Chinese giant salamander (Andrias davidianus) are either completely or largely dependent on hormonal induction. Commercial production of African clawed toads (Xenopus spp.) and also numerous other species rely on hormonal ...

Preparation and use of Xenopus egg extracts to study DNA replication and chromatin associated proteins

The use of cell-free extracts prepared from eggs of the South African clawed toad, Xenopus laevis, has led to many important discoveries in cell cycle research. These egg extracts recapitulate the key nuclear transitions of the eukaryotic cell cycle in vitro under apparently the same controls that exist in vivo. DNA added to the extract is first assembled into a nucleus and is then efficiently ...

The transmission of lymphosarcoma in Xenopus laevis, the South African clawed toad.

The Xenopus lymphosarcoma agent is resistant to freezingthawing, freeze-drying, and antibiotics, but sensitive to heat, ether, and chloroform. It passes a 100-nui filter, and remains in the supernatant fluid following centrifugaron at 30,000 X g for 1 hr. The agent is passed from toad to toad through the water in which the Xenopus live. Phytohemagglutinin injection did not alter the latent peri...