HPLC Method For Determination Of Ramipril

A simple, fast, accurate, precise and reproducible Reversed Phase High Pressure Liquid Chromatography (RPHPLC) method is developed, validated for estimation of ramipril in tablet dosage form. Ramipril is an Angiotension Converting Enzymes (ACE) inhibitor, an anti-hypertensive drug. RP-HPLC is carried on Waters 2695 separation modules, having photo diode array detector. The separation is achieved using Hypersil Octadecyl Silane (ODS) column in isocratic mode with mobile phase, Phosphate buffer: Acetonitrile (30:70v/v), at a flow rate of 2.0 ml/min. Detection is carried out at 210.0 nm. The retention time (RT) for ramipril is about 3.0 ± 0.1minutes. The RP-HPLC method is validated with respect to specificity, accuracy, precision, linearity range, limit of detection (LOD), limit of quantification (LOQ), system suitability studies, robustness, and ruggedness with intraday and interday variation studies. There is no interference from the excipients present in the tablets of ramipril. The plot ratio of area vs concentration of ramipril is found to be linear in the range of 250 μg/ml to 650 μg/ml with coefficient of correlation r =0.999. Recovery experiments are carried out and give results between 97 to 97.86%. The LOD and LOQ is found to be 0.5 μg/ml and 1 μg/ml respectively.