Use of an antisense RNA strategy to investigate the functional significance of Mn-catalase in the extreme thermophile Thermus thermophilus.
نویسندگان
چکیده
The expression of an antisense RNA revealed that an Mn-catalase was required in Thermus thermophilus for aerobic but not for anaerobic growth. The antisense system is based on the constitutive expression of a "bicistronic" transcript consisting of the kanamycin resistance gene mRNA followed by the antisense RNA against the selected target.
منابع مشابه
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 186 22 شماره
صفحات -
تاریخ انتشار 2004