Evolutionary Selection of Bone Mineral Hydroxyapatite Binding Peptides Using Landscape Phage Library
نویسندگان
چکیده
The goal of this work is to identify a peptide that can specifically bind bone mineral hydroxyapatite [(Ca5(PO4)3OH)2] from a phage-displayed random peptide library. Instead of using pIII library where random peptides are displayed at the tip of filamentous phage, we used landscape phage libraries, in which random octa-peptides or nona-peptides are displayed on all the N-termini of the pVIII (major coat protein) of the tetracycline resistant phage (fd-tet). The crystalline hydroxyapatite (HA) powder was used as a target during biopanning. We selected clones that bind with high affinity to HA. Three 8-mer and two 9mer peptides were found to bind to HA with high affinity. The motif alignments showed that the selected binding peptides share common motifs with proteins found in bone including collagen, biglycan, osteocalcin, and osteonectin. Our results indicate that both collagen and non-collagenous proteins can contribute to HA binding during bone formation.
منابع مشابه
Identification of a Novel Tumor-Binding Peptide for Lung Cancer Through in-vitro Panning
Tumor-targeted therapies are playing growing roles in cancer research. The exploitation of these powerful therapeutic modalities largely depends on the discovery of tumor-targeting ligands. Phage display has proven a promising high throughput screening tool for the identification of novel specific peptides with high binding affinity to cancer cells. In the present study, we describe the use of ...
متن کاملEvolutionary screening of collagen-like peptides that nucleate hydroxyapatite crystals.
The biogenesis of inorganic/organic composite materials such as bone typically involves the process of templated mineralization. Biomimetic synthesis of bone-like materials therefore requires the development of organic scaffolds that mediate mineralization of hydroxyapatite (HAP), the major inorganic component of bone. Using phage display, we identified a 12-residue peptide that bound to single...
متن کاملPuzzling Peptides from a Phage Display Library
The commercial availability of random peptide libraries displayed on the M13 phage is increasing their use forstudies on epitope identification, enzyme inhibitors, receptor ligands, etc. In this study two experimentswhere planned for selection of peptides. First with sheep antibodies, the positive selector was IgG, preparedon Protein G column from a pool of 11 sheeps immunized...
متن کاملIdentification of a Novel Tumor-Binding Peptide for Lung Cancer Through in-vitro Panning
Tumor-targeted therapies are playing growing roles in cancer research. The exploitation of these powerful therapeutic modalities largely depends on the discovery of tumor-targeting ligands. Phage display has proven a promising high throughput screening tool for the identification of novel specific peptides with high binding affinity to cancer cells. In the present study, we describe the use of ...
متن کاملDiscovery of Novel Peptidomimetics for Brain-Derived Neurotrophic Factor using Phage Display Technology
Brain-Derived Neurotrophic Factor (BDNF) is a neuroprotectant candidate for neurodegenerative diseases. However, there are several clinical concerns about its therapeutic applications. In the current study, we selected BDNF-mimicking small peptides from phage-displayed peptide library as alternative molecules to the clinical challenges. The peptide library was screened against BDNF receptor (Ne...
متن کامل