Cyclooxygenase-2 inhibits TNFα-mediated apoptosis in renal glomerular mesangial cells
نویسندگان
چکیده
Cyclooxygenase-2 (COX-2) Renal mesangial cells (RMC) Wild type (WT) Adenovirus (Ad) Tumour necrosis factor α (TNFα) Endothelin-1 (ET-1) Interleukin 1β (IL-1β) Prostaglandin E2 and prostaglandin I2 (PGE2 and PGI2) Annexin V (AV) Fluorescein isothiocyanate (FITC) Propidium iodide (PI) Viable (V), early membrane intact apoptotic (EA) and necrotic (N) Hanks Buffered Saline Solution (HBBS) Phosphate buffered saline (PBS) Multiplicity of infection (MOI) Green Fluorescent Protein (GFP) Acridine Orange (AO) Positive (+), negative (-) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)
منابع مشابه
Cyclooxygenase-2 rescues rat mesangial cells from apoptosis induced by adriamycin via upregulation of multidrug resistance protein 1 (P-glycoprotein).
Cyclooxygenase-2 (COX-2) is constitutively expressed in restricted subpopulations of kidney cells, where it presumably acts as an antiapoptotic factor. In conditions that are characterized by inflammation, COX-2 expression also has been described in glomerular mesangial cells (GMC), where COX-2 is not expressed constitutively. It was shown previously that adenovirus-mediated gene transfer of CO...
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