Determinationof /3-Choriogonadotropin in HumanPlasma:Evaluationand Comparisonof Five “Kit” Methods
نویسندگان
چکیده
We evaluated five quantitative, commercially available radioimmunoassay methods (Beta-Tec, BlO-RIA, Cambridge Nuclear, Mallinckrodt, and Serono) for rapid determination of the beta-subunit of human choriogonadotropin in serum or plasma. Only three of the kits (Beta-Tec, Mallinckrodt, and Serono) were found suitable for reliable determination of low concentrations of this analyte, which is of importance in the early diagnosis of trophoblastic disease in men and monitoring effectiveness of chemotherapy of trophoblastic tumors in men and women.
منابع مشابه
Discordant inter-kit results in the radioimmunoassay for choriogonadotropin in serum.
Discordant results for concentrations of choriogonadotropin (HCG) in sera from five of 162 women are reported, as determined with four commercial radioassay kits: Becton-Dickinson, Clinetics, Corning, and Hybritech. Results ranged from 11.2 to 36.7 int. units/L, when assayed with the Becton-Dickinson kit, but HCG was not detectable when assayed with the other kits. None of the patients was preg...
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We describe a visual assay for rapid detection of choriogonadotropin (hGC) in human urine, and evaluate a pregnancy test kit that is based on this assay and designed for use by the general public. The assay involves the formation of an antibody-antigen complex between the anti-hGC antibodies coated on the membrane protein of Staphylococcus aureus bacteria, prestained with hematoxylin, and the h...
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We compared 10 commercially available radioimmunoassay kits (American Diagnostics, Becton Dickinson, BioGenex, Clinical Assays, Hybritech, Leeco, Mallinckrodt, Microanalytic, Nuclear Medical Systems, and Radioassay Systems) for determination of human choriogonadotropin (hCG), using serum pools, hCG CR119 (NIH), 2nd I.S. (who), and 1st I.R.P. (who). Criteria were ease of performance, total assay...
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