EspFU, an Enterohemorrhagic E. Coli Secreted Effector, Hijacks Mammalian Actin Assembly Proteins by Molecular Mimicry and Repetition: A Dissertation
نویسنده
چکیده
ESPFU, AN ENTEROHEMORRHAGIC E. COLI SECRETED EFFECTOR, HIJACKS MAMMALIAN ACTIN ASSEMBLY PROTEINS BY MOLECULAR MIMICRY AND REPETITION
منابع مشابه
Interaction between enterohemorrhagic Escherichia coli O157:H7 EspFu and IRSp53 induces dynamic membrane remodeling in epithelial cells.
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 induces the formation of filamentous, actin-rich, pedestal-shaped structures beneath bacterial cells that have attached to intestinal epithelial cells. Pedestal formation requires the translocation of EHEC O157:H7 type III effectors. One of these type III effectors, EspFu, consists of an N-terminal signal sequence, which is necessary for the tra...
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Upon infection of mammalian cells, enterohemorrhagic E. coli (EHEC) O157:H7 utilizes a type III secretion system to translocate the effectors Tir and EspF(U) (aka TccP) that trigger the formation of F-actin-rich 'pedestals' beneath bound bacteria. EspF(U) is localized to the plasma membrane by Tir and binds the nucleation-promoting factor N-WASP, which in turn activates the Arp2/3 actin assembl...
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Enteropathogenic and enterohemorrhagic Escherichia coli (EPEC and EHEC) are closely-related pathogens that attach tightly to intestinal epithelial cells, efface microvilli, and promote cytoskeletal rearrangements into protrusions called actin pedestals. To trigger pedestal formation, EPEC employs the tyrosine phosphorylated transmembrane receptor Tir, while EHEC relies on the multivalent scaffo...
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Enterohemorrhagic Escherichia coli (EHEC) generate F-actin-rich adhesion pedestals by delivering effector proteins into mammalian cells. These effectors include the translocated receptor Tir, along with EspF(U), a protein that associates indirectly with Tir and contains multiple peptide repeats that stimulate actin polymerization. In vitro, the EspF(U) repeat region is capable of binding and ac...
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M any pathogenic bacteria modulate the host actin assembly machinery to promote infection and spread. Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is a food-borne pathogen that causes severe diarrhea that can be accompanied by hemolytic uremic syndrome with potentially fatal consequences. Pathogenesis of EHEC and a closely-related pathogen, enteropathogenic E. coli (EPEC), is associated wi...
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