Hematological and gas analyzer with acid base balance of pneumoperitoneum in dogs exposed to Laparoscopy

This study was created to investigate the effects of CO2 insufflationinduced pneumoperitoneum on indices of acidbase status and blood picture in dogs. Twelve adult male dogs were subjected to pneumoperitoneum after general anesthesia, pneumoperitoneum was induced throughout the 60 minutes and blood samples were collected at intervals, 0, 5, 15, 30 and 60 minutes. Results of statistical analysis revealed that pneumoperitoneum disturb the acidbase homeostasis by increasing (p≤0. 05) both pCO2, tCO2 and HCO3 whereas pO2 was reduced with no significance on blood pH. Electrolytes including k + and Ca 2+ were significantly increased as a result of pneumoperitoneum yet Na + was relatively stable. Total WBC count, granulocytes and platelets were reduced (p≤0.05) whereas no significance observed related to RBCs, Hb, MCV, MCHC, MCH and Hct. In conclusion, CO2mediated pneumoperitoneum alters acidbase status predisposing to respiratory and metabolic acidosis as well as disturbing immune response.