The cDNA cloning of a pea (Pisum sativum) seed lipoxygenase. Sequence comparisons of the two major pea seed lipoxygenase isoforms.
نویسندگان
چکیده
Cloning and sequencing of two cDNAs from mRNA of maturing pea (Pisum sativum) seeds allowed the deduction of the complete amino acid sequence of a lipoxygenase polypeptide which is most similar to that of soya-bean lipoxygenase 2. The predicted Mr of this polypeptide is 97134, and its sequence permits comparisons between the lox2-type and the lox3-type lipoxygenase isoforms from pea and soya bean (Glycine max).
منابع مشابه
Characterization of authentic recombinant pea-seed lipoxygenases with distinct properties and reaction mechanisms.
The two major isoforms of lipoxygenase (LOX-2 and LOX-3) from pea (Pisum sativum L. cv. Birte) seeds have been cloned and expressed from full-length cDNAs as soluble, active, non-fusion proteins in Escherichia coli. A comparison of both isoforms purified to apparent homogeneity from E. coli and pea seeds has confirmed the authenticity of the recombinant products and established the properties o...
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The sequence of a gene encoding convicilin, a seed storage protein in pea (Pisum sativum L.), is reported. This gene, designated cvcA, is one of a sub-family of two active genes. The transcription start of cvcA was mapped. Convicilin genes are expressed in developing pea seed cotyledons, with maximum levels of the corresponding mRNA species present at 16-18 days after flowering. The gene sequen...
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 264 3 شماره
صفحات -
تاریخ انتشار 1989