ARTHROPOD BIOLOGY Wolbachia Infection in the Coffee Berry Borer (Coleoptera: Scolytidae)

نویسندگان

  • FERNANDO E. VEGA
  • PABLO BENAVIDES
  • JEFFREY A. STUART
  • SCOTT L. O’NEILL
چکیده

A nested polymerase chain reaction protocol yielded positive detection of the maternally inherited cytoplasmic proteobacterium Wolbachia in total genomic DNA from coffee berry borers collected in Benin, Brazil, Colombia, Ecuador, El Salvador, Honduras, India, Kenya, Mexico, Nicaragua, and Uganda. Wolbachia was not detected in specimens from Cameroon, the Dominican Republic, Indonesia, Jamaica, and Peru. AmpliÞed bands from India and Brazil were cloned and sequenced. The 438-bp sequence clearly conformed to Wolbachia group B and was nearly identical to that of Ephestia kuehniella. The possible implications of Wolbachia infection in the coffee berry borer are discussed.

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تاریخ انتشار 2002