MERCK Ion Exchange Chromatography Using Fractogel EMD Tentacle Supports
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Removal of Aggregates of Monoclonal Antibodies by Ion Exchange Chromatography
Abstract—The primary objective of this work was to study the effect of resin chemistry, pH and molarity of binding and elution buffer on aggregate removal using Cation Exchange Chromatography and find the optimum conditions which can give efficient aggregate removal with minimum loss of yield. Four different resins were used for carrying out the experiments: Fractogel EMD SO3(S), Fractogel EMD ...
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