Use of Salmonella phage P22 for transduction in Escherichia coli.
نویسندگان
چکیده
A cosmid (pPR1347) carrying both the rfb gene cluster and the rfc gene of a Salmonella group B serovar has been constructed; Escherichia coli K-12 strains carrying this cosmid produce long-chain O antigen, are sensitive to phage P22, and can be transduced by P22. Some of the benefits of P22 transduction are now available for studying E. coli and potentially other genera.
منابع مشابه
Transduction of various R factors by phage P1 in Escherichia coli and by phage P22 in Salmonella typhimurium.
R factors fi(+) and fi(-), with various combinations of drug-resistance markers and isolated from independent sources, were transduced by phage P1kc in Escherichia coli and by phage P22 in Salmonella typhimurium. Usually the entire R factor was transduced by P1kc in E. coli, as indicated by the absence of segregation of the drug-resistance markers from their conjugal transferability. In contras...
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Salmonella phage P22 has been used in the construction of three sorts of specialized transducing phage: P22 proAB, P22 proABlac and P22 argF. The bacterial genes carried are derived from E. coli K12. Since E. coli and Salmonella chromosomes recombine very poorly, E. coli genes cannot be transduced into Salmonella recipients by P22‘s generalized transduction mechanism. Therefore, stable inherita...
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Superinfection immunity is found in the conjugal transfer of R factors between two fi(+) R factors and between two fi(-) R factors (fi = fertility inhibition), as we reported previously. In contrast, no reduction in the frequencies of transduction of an fi(+) R factor 222 was caused by the presence of fi(+) R factors in the recipients in transduction systems with phage P1kc in Escherichia coli ...
متن کاملTransfection of Escherichia coli and Salmonella typhimurium Spheroplasts: Host-Controlled Restriction
Under proper conditions, one infective center was obtained for 3 X 108 molecules of P22 phage deoxyribonucleic acid (DNA) when lysozyme-ethylenediaminetetraacetic acid spheroplasts of Escherichia coil were transfected in the presence of 25 /Ig of protamine sulfate per ml. A 3to 50-fold B-specific and K-specific E. coli restriction of the incoming P22 DNA was observed. When P22 DNA-infected E. c...
متن کاملTransfection of Escherichia coli and Salmonella typhimurium Spheroplasts: Host-Controlled Restriction
Under proper conditions, one infective center was obtained for 3 X 108 molecules of P22 phage deoxyribonucleic acid (DNA) when lysozyme-ethylenediaminetetraacetic acid spheroplasts of Escherichia coil were transfected in the presence of 25 /Ig of protamine sulfate per ml. A 3to 50-fold B-specific and K-specific E. coli restriction of the incoming P22 DNA was observed. When P22 DNA-infected E. c...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 175 21 شماره
صفحات -
تاریخ انتشار 1993