Evaluation of a rapid method for identifying Branhamella catarrhalis.
نویسنده
چکیده
The speed and precision of hydrolysis of tributyrin to butyric acid as a test to detect Branhamella catarrhalis were evaluated. The test proved consistently reliable in the identification of strains and correctly differentiated B catarrhalis from Neisseria sp. The combination of Gram stain, oxidase, and catalase tests to tributyrin hydrolysis provides a means of positive same day identification of B catarrhalis.
منابع مشابه
Rapid method for differentiating strains of Branhamella catarrhalis.
The ability of Branhamella catarrhalis to cause nosocomial infections is a matter of some controversy. The API ZYM research kit for detecting 89 enzymes was used on 49 isolates of B catarrhalis to select enzymes of potential use in differentiating clinical isolates. Twenty nine enzymes were produced by all isolates (13 strongly positive) and many of these were esterases; 16 enzymes were not det...
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Branhamella catarrhalis, an occasional cause of human respiratory infections, unlike most other members of the family Neisseriaceae, produces a butyrate esterase. This is capable of breaking the ester linkage between butyryl groups and carrier molecules. B. caviae and B. ovis, which are rarely encountered in pathological specimens, also produce butyrate esterase. This property can be used as a ...
متن کاملB.CAT CONFIRM, a rapid test for confirmation of Branhamella catarrhalis.
B.CAT CONFIRM (Scott Laboratories, Inc., Fiskeville, R.I.), a rapid test for detection of tributyrin hydrolysis, was evaluated for its ability to identify strains of Branhamella catarrhalis and to differentiate them from Neisseria species and related species. On initial testing, B.CAT CONFIRM was positive for 65 (96%) of 68 B. catarrhalis strains within 30 min after inoculation. Retesting of th...
متن کاملEvaluation of the RIM-N, Gonochek II, and Phadebact systems for the identification of pathogenic Neisseria spp. and Branhamella catarrhalis.
Methods for identifying Neisseria spp. include conventional and modified carbohydrate degradation procedures, chromogenic enzyme substrate tests, and immunologic coagglutination tests for Neisseria gonorrhoeae. In this study, we evaluated the abilities of the RIM-N carbohydrate degradation system (American MicroScan, Campbell, Calif.), the Gonochek II enzymatic identification system (Du Pont Co...
متن کاملPneumonia due to Branhamella catarrhalis.
In 12 of 451 patients diagnosed as having pneumonia in a single hospital over 18 months the causative organism appeared to be Branhamella catarrhalis.
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ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 41 4 شماره
صفحات -
تاریخ انتشار 1988