Automating the application of smart materials for protein crystallization
نویسندگان
چکیده
The fabrication and validation of the first semi-liquid nonprotein nucleating agent to be administered automatically to crystallization trials is reported. This research builds upon prior demonstration of the suitability of molecularly imprinted polymers (MIPs; known as `smart materials') for inducing protein crystal growth. Modified MIPs of altered texture suitable for high-throughput trials are demonstrated to improve crystal quality and to increase the probability of success when screening for suitable crystallization conditions. The application of these materials is simple, time-efficient and will provide a potent tool for structural biologists embarking on crystallization trials.
منابع مشابه
CRYSTALLIZATION AND SINTERABILITY BEHAVIOR OF BIORESORBABLE CaO-P2O5-Na2O-TiO2 GLASS CERAMICS FOR BONE REGENERATION APPLICATION
Abstract:Some types of glass and glass ceramics have a great potential for making bone tissue engineering scaffolds, drug carrier and bone cements as they can bond to host bone, stimulate bone cells toward osteogenesis, and resorb at the same time as the bone is repaired. Calcium phosphate glass ceramics have very attractive properties that allow them to use in bone tissue engineering. Calcium ...
متن کاملKinetics of Ceramic Phase Crystallization in a Glass Derived from Wastes of Iron and Steel Industry
Intensified environmental regulations have posed numerous challenges in the disposal of industrial wastes. The steel industry is one of the biggest production industries, with a considerable amount of daily wastes. Production of glass-ceramic from the steel industry waters is one of the proper solutions for this problem. In this study, the application utilization of different wastes (such as bl...
متن کاملProtein crystallization facilitated by molecularly imprinted polymers.
We present a previously undescribed initiative and its application, namely the design of molecularly imprinted polymers (MIPs) for producing protein crystals that are essential for determining high-resolution 3D structures of proteins. MIPs, also referred to as "smart materials," are made to contain cavities capable of rebinding protein; thus the fingerprint of the protein created on the polyme...
متن کاملApplications of peptide and protein-based materials in bionanotechnology.
In this critical review we highlight recent advances in the use of peptide- and protein-related materials as smart building blocks in nanotechnology. Peptides and proteins can be very practical for new material synthesis and device fabrications. For example, peptides and proteins have superior specificity for target binding as seen in the antibody recognition and this biological recognition fun...
متن کاملIMPROVED CARBON BONDED MGO MATERIALS AS SMART REFRACTORIES
Improved structures of MgO carbon bonded materials due to new binder systems and due to the application of electrical currents during operation have been achieved for advanced applications in the secondary metallurgy and during near net shape metal casting.
متن کامل