Fluorophotometric Apparatus for Tie Objective Determination of Fluorescence in Tie Anterior Chamber of the Living Eye*

FOR many years considerable attention has been focused on the problem of the origin and circulation ofthe aqueous humour under normal and pathological conditions. One of the methods applied to this study is the detection within the eye of dyes which enter the anterior chamber across the bloodaqueous baffler-a method first suggested by Ehrlich (1882) when he observed that fluorescein injected intravenously passes into the aqueous humour. Ehrlich anticipated that the use of this dye would promote a deeper understanding of the problem, and many ways have since been devised of determining the presence of fluorescein in the eye of the living animal. In the present paper an instrument is described which permits an objective determination of the degree of fluorescence under experimental and clinical conditions.