Purification of mouse encephalomyelitis virus (GDVII virus) by means of ether extraction, acetone precipitation, trypsin digestion, and hemagglutination.

In the previous papers, some attempts for purification and concentration of the GDVII strain of mouse encephalomyelitis virus by means of ether extraction, metanol-acetone precipitation, hemagglutination , and agglutination to red cell stroma were reported. Purification of the GDVI virus by hemagglutination was described by Lahelle and Ward. The presenting paper is to describe a method for purification of the GDVII virus and to show an electron micrograph of the virus particles. In particular, the experiments payed an attention to removal of normal tissue components contained in the starting materials.