An electrochemical tyrosinase-immobilized biosensor for albumin—toward a potential total protein measurement
نویسندگان
چکیده
A simple biosensor, based on a tyrosinase-immobilized electrode, was developed for a rapid and quantitative measurement of bovine serum albumin (BSA) and human serum albumin (HSA). Tyrosinase, immobilized on a screen-printed carbon electrode, catalyzed the oxidation of tyrosine residues in the albumin to the corresponding quinone, which was further reduced electrochemically by the sensing electrode under an appropriate condition. The concentration of the protein, therefore, could be quantified by measuring the reduction current. The operational p o n ©
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