Crystalline beef kidney rhodanese.

Although rhodanese activity occurs in many tissues (l-3), the enzyme had been obtained in crystalline form from beef liver only (4). Beef kidney rhodanese could not be crystallized by the same procedure (5). To extend the biochemical genetic comparison reported previously (5), however, it was desired to obtain the kidney enzyme in a very high state of purity. This paper reports the purification and crystallization of beef kidney rhodanese by a method which combines an ion exchange fractionation with steps based on Siirbo’s procedure for the crystalline liver enzyme (4). The same method has also been used for preparing crystalline beef liver rhodanese. Some further comparative data are presented.