Tungstate administration improves the sexual and reproductive function in female rats with streptozotocin-induced diabetes.

نویسندگان

  • J Ballester
  • M C Muñoz
  • J Domínguez
  • M J Palomo
  • M Rivera
  • T Rigau
  • J J Guinovart
  • J E Rodríguez-Gil
چکیده

BACKGROUND Diabetes induces great alterations in female reproductive function. We analyzed the effects of tungstate, an anti-diabetic agent, on the reproductive function of healthy and diabetic female rats. METHODS Healthy and streptozotocin-induced diabetic rats were treated with sodium tungstate (2 mg/ml in their drinking water) for 12 weeks. Markers of reproductive function and diabetes were measured in serum, and in uterus and ovaries by Western blot or RT-PCR. Reproductive function was also assessed by mating. RESULTS Diabetic rats showed great impairment of libido, which was accompanied by a total loss of fertility (P < 0.05) and a decrease in the serum levels of FSH (P < 0.05) and LH (P < 0.05) compared with healthy rats. Tungstate treatment of diabetic rats partially recovered libido while fertility rate increased to 66.6%. This improvement was accompanied by a recovery of serum FSH (to a level higher than healthy rats) and LH. Moreover, tungstate treatment normalized ovarian expression of GLUT 3 hexose transporter, and estrogen, progesterone and FSH receptors, whereas only GLUT 3 and FSH receptors were normalized in the uterus. CONCLUSIONS Our results indicate that the alterations in female reproduction in diabetes were partially reversed after tungstate treatment by a mechanism(s) involving the normalization of serum FSH and LH levels, and ovarian and uterine expression of FSH receptors and GLUT3.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Beta Cell Protective Effects of Sodium Tungstate in Streptozotocin-Induced Diabetic Rats: Glycemic Control, Blockage of Oxidative Stress and Beta Cell Histochemistry

Background: Diabetes is a major public health problem. The development of new therapies that are able to improve glycemia management and even to cure diabetes is of great interest. In this study, protective effects of sodium tungstate against STZ-induced beta-cell damages were investigated. Methods: Sixty rats were divided into six groups: control, diabetic, sodium tungstate treated diabetic r...

متن کامل

Biochemical and Histological Study of Protective Effect of Sodium Tungstate on Oxidative Stress Induced by Streptozotocin in Pancreas of Diabetic Rats

Purpose: The aim of this study was evaluation of effects of sodium tungstate on oxidative stress in pancreas of streptozotocin induced diabetic rats. Materials and Methods: Sixty mature Wistar male rats (Age: 2-3 month) were selected and divided into six groups randomly (n=10). Control (C), STZ-induced diabetic (D), STZ-induced diabetic rats were treated by sodium tungstate (1 mg/ml) in drinkin...

متن کامل

The effect of saffron aqueous extract on oxidative stress parameters and important biochemical enzymes in the testis of streptozotocin-induced diabetic rats

Introduction: Sexual dysfunction and infertility are frequently associated with diabetes in men and experimental animals. Oxidative stress and alteration in testis are responsible for complication in diabetes. Saffron has antidiabetic and antioxidant properties that improves the functions of various organs. Therefore, the aim of the present study was to investigate the effects of administra...

متن کامل

Tungstate treatment improves Leydig cell function in streptozotocin-diabetic rats.

Oral administration of sodium tungstate to adult male streptozotocin-diabetic rats for 3 months normalized serum levels of glucose, insulin, luteinizing hormone, and follicle-stimulating hormone. These effects were accompanied by an increase in reproductive performance, which was related to a strong improvement in Leydig cell function markers, such as the recovery of the number of Leydig cells ...

متن کامل

Aqueous extract of Berberis integerrima root improves renal dysfunction in streptozotocin induced diabetic rats

Objective: Barberry root extract contains various alkaloids that are considered as antioxidants. Beneficial effect of aqueous extract of Berberis integerrima root (AEBIR) was evaluated for renal function in diabetic rats induced by STZ. Material and Methods: Diabetes was induced by i.p. injection of streptozotocin (65 mg/kg bw) to rats, after 15 h of fasting. Diabetic rats were randomly grouped...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Human reproduction

دوره 22 8  شماره 

صفحات  -

تاریخ انتشار 2007