Quantitative RT-PCR: limits and accuracy.

Nanoliter high throughput quantitative PCR

Understanding biological complexity arising from patterns of gene expression requires accurate and precise measurement of RNA levels across large numbers of genes simultaneously. Real time PCR (RT-PCR) in a microtiter plate is the preferred method for quantitative transcriptional analysis but scaling RT-PCR to higher throughputs in this fluidic format is intrinsically limited by cost and logist...

Development and Validation of an Externally Standardised Quantitative Insulin-like Growth Factor-1 RT-PCR Using LightCycler

RT-PCR has a detection limit 10–100 fold lower than protection-assay or northern hybridisation, respectively. The RT-ribonuclease PCR quantification technique of choice depends on the target sequence, the expected range of the mRNA amount present in the tissue, the degree of accuracy required, and whether quantification needs to be relative or absolute. Externally standardised RT-PCR with quant...

Reliable gene expression analysis by reverse transcription-quantitative PCR: reporting and minimizing the uncertainty in data accuracy.

Reverse transcription-quantitative PCR (RT-qPCR) has been widely adopted to measure differences in mRNA levels; however, biological and technical variation strongly affects the accuracy of the reported differences. RT-qPCR specialists have warned that, unless researchers minimize this variability, they may report inaccurate differences and draw incorrect biological conclusions. The Minimum Info...

short Communication: Optimization of semi-quantitative RT PCR analysis for CPT I gene expression in Rainbow trout (Oncorhynchus mykiss)

Comparing the Diagnostic Power of Chest CT Scan and RT-PCR in Diagnosis of COVID-19

Background and purpose: Proper diagnosis of patients with COVID-19 is one of the challenges in medical centers. RT-PCR is the standard and reference test in diagnosis of patients with COVID-19. This study aimed at accelerating the correct diagnosis of COVID-19 and the diagnostic power of chest CT scan and RT-PCR. Materials and methods: This study was performed in 569 patients with COVID-19 adm...