Reply: complexity in camalexin biosynthesis.

are degraded by g-glutamyl transpeptidase GGT3 in Arabidopsis. Plant J. 49: 878–888. Parisy, V., Poinssot, B., Owsianowski, L., Buchala, A., Glazebrook, J., and Mauch, F. (2007). Identification of PAD2 as a g-glutamylcysteine synthetase highlights the importance of glutathione in disease resistance of Arabidopsis. Plant J. 49: 159–172. Saga, H., Ogawa, T., Kai, K., Suzuki, H., Ogata, Y., Sakurai, N., Shibata, D., and Ohta, D. (2012). Identification and characterization of ANAC042, a transcription factor family gene involved in the regulation of camalexin biosynthesis in Arabidopsis. Mol. Plant Microbe Interact. 25: 684–696. Saito, K., Hirai, M.Y., and Yonekura-Sakakibara, K. (2008). Decoding genes with coexpression networks and metabolomics ‘Majority report by precogs’. Trends Plant Sci. 13: 36–43. Schuhegger, R., Nafisi, M., Mansourova, M., Petersen, B.L., Olsen, C.E., Svatos, A., Halkier, B.A., and Glawischnig, E. (2006). CYP71B15 (PAD3) catalyzes the final step in camalexin biosynthesis. Plant Physiol. 141: 1248–1254. Schuler, M.A., and Werck-Reichhart, D. (2003). Functional genomics of P450s. Annu. Rev. Plant Biol. 54: 629–667. Su, T.B., Xu, J.A., Li, Y.A., Lei, L., Zhao, L., Yang, H.L., Feng, J.D., Liu, G.Q., and Ren, D.T. (2011). Glutathione-indole-3-acetonitrile is required for camalexin biosynthesis in Arabidopsis thaliana. Plant Cell 23: 364–380. Wada, K., Hiratake, J., Irie, M., Okada, T., Yamada, C., Kumagai, H., Suzuki, H., and Fukuyama, K. (2008). Crystal structures of Escherichia coli g-glutamyltranspeptidase in complex with azaserine and acivicin: novel mechanistic implication for inhibition by glutamine antagonists. J. Mol. Biol. 380: 361–372. Wang, M.-Y., Liu, X.-T., Chen, Y., Xu, X.-J., Yu, B., Zhang, S.-Q., Li, Q., and He, Z.-H. (2012). Arabidopsis acetyl-amido synthetase GH3.5 involvement in camalexin biosynthesis through conjugation of indole-3-carboxylic acid and cysteine and upregulation of camalexin biosynthesis genes. J. Integr. Plant Biol. 54: 471–485. Weng, J.-K., Philippe, R.N., and Noel, J.P. (2012). The rise of chemodiversity in plants. Science 336: 1667–1670. Winter, D., Vinegar, B., Nahal, H., Ammar, R., Wilson, G.V., and Provart, N.J. (2007). An “Electronic Fluorescent Pictograph” browser for exploring and analyzing largescale biological data sets. PLoS ONE 2: e718. Zhou, N., Tootle, T.L., and Glazebrook, J. (1999). Arabidopsis PAD3, a gene required for camalexin biosynthesis, encodes a putative cytochrome P450 monooxygenase. Plant Cell 11: 2419–2428. Ziegler, J., and Facchini, P.J. (2008). Alkaloid biosynthesis: Metabolism and trafficking. Annu. Rev. Plant Biol. 59: 735–769.