CELL DIFFERENTIATION AND PROLIFERATION T Cadherin Suppresses the Cell Proliferation of Mouse Melanoma B16F10 and Tumor Angiogenesis

The influence of T cadherin on the pigmentation and proliferation of mouse melanoma B16F10 cells in vitro and on the growth and neovascularization of tumor cell masses formed by the B16F10 cells in a model of the chorioallantoic membrane of a chicken embryo is studied. It is found that the proliferative activity of the cells decreases in the cell culture of mouse melanoma upon the overexpression of T cadherin in comparison with the cells in the control. It is shown in experiments in vitro that the B16F10 cells with the overexpression of T cadherin are rarely settling are to the chorioallantoic membrane than the control cells. In addition, it is found that the con trol cells of mouse melanoma form tumors with area more 0.1 mm2 more often than the cells with the overexpres sion of T cadherin and the amount of the vessels growing to tumor cell masses formed by the cells with the overex pression of T cadherin is significantly lower than the same index for the cells in the control. Thus, the overexpres sion of T cadherin in the B16F10 cells suppresses the proliferation of these cells in vitro and the growth of the tumor masses formed by melanoma cells on the chorioallantoic membrane and their neovascularization in vivo.