Supporting Online Material for DNA Binding Site Sequence Directs Glucocorticoid Receptor Structure and Activity
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Sequences flanking the core-binding site modulate glucocorticoid receptor structure and activity
The glucocorticoid receptor (GR) binds as a homodimer to genomic response elements, which have particular sequence and shape characteristics. Here we show that the nucleotides directly flanking the core-binding site, differ depending on the strength of GR-dependent activation of nearby genes. Our study indicates that these flanking nucleotides change the three-dimensional structure of the DNA-b...
متن کاملDNA binding site sequence directs glucocorticoid receptor structure and activity.
Genes are not simply turned on or off, but instead their expression is fine-tuned to meet the needs of a cell. How genes are modulated so precisely is not well understood. The glucocorticoid receptor (GR) regulates target genes by associating with specific DNA binding sites, the sequences of which differ between genes. Traditionally, these binding sites have been viewed only as docking sites. U...
متن کاملCorrigendum: Sequences flanking the core-binding site modulate glucocorticoid receptor structure and activity
The glucocorticoid receptor (GR) binds as a homodimer to genomic response elements, which have particular sequence and shape characteristics. Here we show that the nucleotides directly flanking the core-binding site, differ depending on the strength of GR-dependent activation of nearby genes. Our study indicates that these flanking nucleotides change the three-dimensional structure of the DNA-b...
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Background: Androgen receptor (AR) plays a critical role in prostate cancer and male sexual differentiation.Mechanisms by which AR acts and regulations of AR nucleocytoplasmic shuttling are not understood well. Materials and Methods: Degenerate PCR and RACE Cloning of AR Gene; Phylogenetic Analysis and Molecular Modeling;Real-time Fluorescent Quantitative RT-PCR; Northern Blot Hybridization;In ...
متن کاملStructural Variants of Binding Sites for Glucocorticoid Receptor and the Mechanisms of Glucocorticoid Regulation: Analysis of Gr-trrd Database
Motivation: Glucocorticoid receptor (GR) is an important regulator of many genes involved in a variety of biochemical and physiological processes. The features of the structure and localization of DNA binding sites for GR may be significant for ensuring specificity of glucocorticoid-mediated regulation of different genes. Results: GR-TRRD database accumulates the largest out of currently publis...
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