Inhibition of Gap Junction–Mediated Intercellular Communication by TNF- in Cultured Human Corneal Fibroblasts
نویسندگان
چکیده
RESULTS. TNFinduced a timeand concentration-dependent decrease in GJIC activity in human corneal fibroblasts. Immunofluorescence analysis revealed that TNFreduced the level of specific staining for Cx43 at sites of contact between adjacent cells. Immunoblot analysis detected four specific Cx43 bands, one corresponding to the nonphosphorylated form of the protein and three corresponding to phosphorylated forms. Exposure of cells to TNFreduced the relative abundance of the three phosphorylated forms of Cx43. The amount of Cx43 mRNA was not affected by TNF.
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