A Micropropagation System for Cloning of Hemp (cannabis Sativa L.) by Shoot Tip Culture

This study describes the standardization of an efficient in vitro propagation and hardening procedure for obtaining plantlets from shoot tips of Hemp (Cannabis sativa L.). Hemp seedlings were germinated on half-strength 1/2 MS medium supplemented with 10 g·Lsucrose, 5.5 g·Lagar at a pH of 6.8 under light for 16 h per day. MS medium containing 0.2 mg·LTDZ, 0.1 mg·LNAA supported the maximal auxiliary bud multiplication rate of 3.22 per shoot tip. The proliferated buds were successfully rooted on MS medium supplemented with 0.1 mg·LIBA and 0.05 mg·LNAA resulting in 85% of the plantlets rooting. The procedure requires a 54 days cycle for the In vitro clonal propagation (14 days for shoot multiplication and 40 days for root induction) which includes 35-42 days for acclimatized plantlet production.