RNasin® Ribonuclease Inhibitor Part I: Characterization of the Protein, PN077
نویسنده
چکیده
Ribonucleases (RNases) are ubiquitous enzymes that can detrimentally affect RNA isolation in the laboratory. Many applications such as RT-PCR and in vitro transcription and translation may be affected as well. RNase can renature after autoclaving and is difficult to inactivate. Many RNase inhibitors are available commercially. Promega’s RNasin® Ribonuclease Inhibitor, one of the first introduced, is exceptionally effective at controlling RNase activity during molecular biology procedures.
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RNasin® Ribonuclease Inhibitor Part II: A Tale of Two Proteins, PN077
In this article we present data that compares RNasin® Ribonuclease Inhibitor to SUPERase•InTM, an RNase inhibitor recently introduced by Ambion. The two RNase inhibitors are compared for their ability to protect RNA from degradation under a variety of different reaction conditions. In addition, we tested both inhibitors for their ability to protect RNA during in vitro translation and transcript...
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