Rapid communication: Thirty-eight polymorphic microsatellite markers for mapping in rainbow trout.

Species. Oncorhynchus mykiss. Source and Description. Microsatellite repeats were identified by sequencing clones from microsatellite-enriched libraries constructed from genomic DNA. Microsatellite markers were developed by designing primers to amplify the repeats. Primer Sequences. See Table 1. Method of Detection. PCR conditions were optimized using rainbow trout DNA (Kamloop strain) and miniprep DNA from the clone used to develop the marker. PCR reactions included 25 ng of DNA, 1.5 to 2 mM MgCl2, 1× manufacturer’s reaction buffer (Applied Biosystems, Foster City, CA), 2 mM dNTPs, 1 M each of forward and reverse primer, and 0.05 units of AmpliTaq Gold (Perkin-Elmer, Norwalk, CT). Reactions were thermocycled as follows: 10 min at 94°C, 35 cycles of 94°C for 30 s, annealing temperature for 30 s, and 72°C for 30 s, and a final extension at 72°C for 5 min. PCR