TECHNICAL ADVANCE Inverted repeat of a heterologous 30-untranslated region for high-efficiency, high-throughput gene silencing
نویسندگان
چکیده
Received 25 September 2002; revised 22 October 2002; accepted 27 November 2002. For correspondence (fax þ6 351 7050; e-mail [email protected]). yPresent address: Crop and Food Research, Private Bag 11600, Palmerston North, New Zealand. zPresent address: Sygen International, Suite 130, 2929 Seventh Street, Berkeley, CA 94710, USA. Present address: Molecular Biology Department, University of Wyoming, Laramie, WY 82070, USA. Present address: Torrey Mesa Research Institute, Syngenta Research and Technology, 3115 Merryfield Row, San Diego, CA 92121, USA. yyPresent address: Mendel Biotechnology, 21375 Cabot Boulevard, Hayward, CA 94545, USA.
منابع مشابه
Comparative bioinformatics analysis of a wild diploid Gossypium with two cultivated allotetraploid species
Background: Gossypium thurberi is a wild diploid species that has been used to improve cultivated allotetraploid cotton. G. thurberi belongs to D genome, which is an important wild bio-source for the cotton breeding and genetic research. To a certain degree, chloroplast DNA sequence information are a versatile tool for species identification and phylogenetic implications in plants. Different ch...
متن کاملUtility of P19 Gene-Silencing Suppressor for High Level Expression of Recombinant Human Therapeutic Proteins in Plant Cells
Background: The potential of plants, as a safe and eukaryotic system, is considered in the production of recombinant therapeutic human protein today; but the expression level of heterologous proteins is limited by the post-transcriptional gene silencing (PTGS) response in this new technology. The use of viral suppressors of gene silencing can prevent PTGS and improve transient expression level ...
متن کاملEfficiency of gene silencing in Arabidopsis: direct inverted repeats vs. transitive RNAi vectors.
We investigated the efficiency of RNA interference (RNAi) in Arabidopsis using transitive and homologous inverted repeat (hIR) vectors. hIR constructs carry self-complementary intron-spliced fragments of the target gene whereas transitive vectors have the target sequence fragment adjacent to an intron-spliced, inverted repeat of heterologous origin. Both transitive and hIR constructs facilitate...
متن کاملDesigning highly effective and genetically invert fragments by full assessment of mutations in seed region siRNAs in omega gliadin epitopes
RNAi mechanism plays a major role in silencing the expression of target genes by siRNAs. In the current study, in silico properties of 30 genes in omega-2 gliadin and 266 nt and 326 nt mutations were investigated before and after cloning in an expression vector. Specific primers were designed for 30 genes with spacer regions of 75 nt and 178 nt (for gene invert repeats). The frequency of siRNA ...
متن کاملP-204: Evaluation of FMR1 Gene Regulatory Region for The Epigenetic Mark of H3K9 Acetylation in Blood Cells of Patients with Diminished Ovarian Reserve Reffered to Royan Institute
Background: Diminished ovarian reserve (DOR) is a heterogeneous disorder causing infertility, characterized by a decreased number of oocytes and high FSH level, the genetic cause of which is still unknown. The association between FMR1 premutations(50-200 CGG repeats) and the premature ovarian failure( POF) disease has suggested that FMR1 gene acts as a risk factor for POF and recently for DOR p...
متن کامل