Unique peptide prediction of RNase family sequences based on reinforced merging algorithms
نویسندگان
چکیده
Human ribonuclease A (RNase A) superfamily consists of eight RNases with high sequence homology, in which RNase2 and RNase3 share 78% similarity. The evolutionary variation of RNases results in differential structure and function of the enzymes. To distinguish the characteristics of each RNase, we developed reinforced merging algorithms (RMA) to rapidly predict and identify the unique sequence motifs for each member of the highly conservative human RNaseA superfamily. Two unique regions in RNase3 were predicted and experimentally confirmed to contain epitopes for monoclonal antibodies (mAbs) specifically against RNase3. Our method provides a useful tool for identification of unique sequence motif for further experimental design.
منابع مشابه
Unique Peptide Identification of Rnasea Superfamily Sequences Based on Reinforced Merging Algorithms
Human ribonuclease A (RNaseA) superfamily consists of eight RNases with high similarity in which RNase2 and RNase3 share 76.7% identity. The evolutionary variation of RNases results in differential structures and functions of the enzymes. To distinguish the characteristics of each RNase, we developed reinforced merging algorithms (RMA) to rapidly identify the unique peptide motifs for each memb...
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