Genome sequence of Azotobacter vinelandii, an obligate aerobe specialized to support diverse anaerobic metabolic processes.
نویسندگان
چکیده
Azotobacter vinelandii is a soil bacterium related to the Pseudomonas genus that fixes nitrogen under aerobic conditions while simultaneously protecting nitrogenase from oxygen damage. In response to carbon availability, this organism undergoes a simple differentiation process to form cysts that are resistant to drought and other physical and chemical agents. Here we report the complete genome sequence of A. vinelandii DJ, which has a single circular genome of 5,365,318 bp. In order to reconcile an obligate aerobic lifestyle with exquisitely oxygen-sensitive processes, A. vinelandii is specialized in terms of its complement of respiratory proteins. It is able to produce alginate, a polymer that further protects the organism from excess exogenous oxygen, and it has multiple duplications of alginate modification genes, which may alter alginate composition in response to oxygen availability. The genome analysis identified the chromosomal locations of the genes coding for the three known oxygen-sensitive nitrogenases, as well as genes coding for other oxygen-sensitive enzymes, such as carbon monoxide dehydrogenase and formate dehydrogenase. These findings offer new prospects for the wider application of A. vinelandii as a host for the production and characterization of oxygen-sensitive proteins.
منابع مشابه
The genome sequence of Azotobacter vinelandii , an obligate 1 aerobe specialized to support diverse anaerobic metabolic 2 processes 3 4 running title : Genome sequence of Azotobacter vinelandii
6 João C. Setubal, Patricia dos Santos, Barry S. Goldman, Helga Ertesvåg, 7 Guadelupe Espin, Luis M. Rubio, Svein Valla, Nalvo F. Almeida, Divya 8 Balasubramanian, Lindsey Cromes, Leonardo Curatti, Zijin Du, Eric Godsy, Brad 9 Goodner, Kaitlyn Hellner-Burris, José A. Hernandez, Katherine Houmiel, Juan 10 Imperial, Christina Kennedy, Timothy J. Larson, Phil Latreille, Lauren S. Ligon, 11 Jing Lu...
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Azotobacter vinelandii cysts undergo conversion to vegetative cells in Burk's nitrogen-free medium utilizing glucose, sucrose, or acetate. In 1% glucose, this overall process was complete in 8 hr and consisted of a germination and an outgrowth phase. Respiration, ribonucleic acid, and protein synthesis began soon after the addition of the germinant, and these processes proceeded at rates charac...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 191 14 شماره
صفحات -
تاریخ انتشار 2009