Mutations in the gyrA, parC, and parE genes associated with fluoroquinolone resistance in clinical isolates of Mycoplasma hominis.
نویسندگان
چکیده
Five clinical isolates of Mycoplasma hominis from three different patients were examined for resistance to fluoroquinolones; some of these isolates were probably identical. All five isolates harbored amino acid substitutions in the quinolone resistance-determining regions of both DNA gyrase (GyrA) and topoisomerase IV (ParC or ParE). Furthermore, the novobiocin MIC for three isolates showed a significant increase. This is the first characterization of fluoroquinolone-resistant clinical mycoplasma isolates from humans.
منابع مشابه
Molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis clinical isolates
To evaluate the molecular mechanism of fluoroquinolones resistance in Mycoplasma hominis (MH) clinical strains isolated from urogenital specimens. 15 MH clinical isolates with different phenotypes of resistance to fluoroquinolones antibiotics were screened for mutations in the quinolone resistance-determining regions (QRDRs) of DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE) in ...
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Objective(s): We investigated the contribution of gyrA and parC mutational mechanism in decreased ciprofloxacin susceptibility of Acinetobacter baumannii isolated from burn wound infections. Materials and Methods: Ciprofloxacin susceptibility of 50 A. baumannii isolates was evaluated by disk diffusion and agar dilution methods. PCR and sequencing were performed for detection of mutation in gyr...
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متن کاملAlterations in topoisomerase IV and DNA gyrase in quinolone-resistant mutants of Mycoplasma hominis obtained in vitro.
Mycoplasma hominis mutants were selected stepwise for resistance to ofloxacin and sparfloxacin, and their gyrA, gyrB, parC, and parE quinolone resistance-determining regions were characterized. For ofloxacin, four rounds of selection yielded six first-, six second-, five third-, and two fourth-step mutants. The first-step mutants harbored a single Asp426-->Asn substitution in ParE. GyrA changes...
متن کاملتعیین الگوی مقاومت آنتیبیوتیکی و بررسی موتاسیون در ژنهای gyrA و parC در سویههای اشریشیاکلی جدا شده از بیماران مبتلا به عفونت ادراری
Introduction & Objective: Fluoroquinolones are essential antimicrobial agents used to treat UTIs. Clinical experiences have shown a high rate of antibiotic resistance among uropathogens. These resistance are usually the consequence of mutations involving genes encoding gyrA and parC. The aim of this study was to determine antimicrobial resistance pattern and the presence of mutations in regions...
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 43 4 شماره
صفحات -
تاریخ انتشار 1999