Production, Purification and Characterization of Protease by Aspergillus flavus under Solid State Fermentation
نویسندگان
چکیده
Protease production under solid state fermentation (SSF) was investigated using isolated Aspergillus flavus. Different agroindustrial waste products were evaluated to check the possibility of potential utilization of substrates in SSF for protease production by Aspergillus flavus using wheat bran as a substrate. The results showed that the optimum conditions for maximum protease production were found to be 7 day of incubation at pH 5.0, temperature 30C; inoculum size 3%; substrate concentration 3% and 3% KNO3 as nitrogen source. The purified enzyme produced 5.8 fold with recovery of 3.2% by DEAE-column chromatography and the molecular weight was estimated to be 46kDa by SDS-PAGE. It has a Vmax value of 60.0 U/mg and Km value of 0.6 mg/ml at pH of 7. The enzyme activity was found to be stable at 50 0 C and it was stimulated by metal ions like Cu and Zn and inhibited by Ca and Mg . © 2011 Jordan Journal of Biological Sciences. All rights reserved
منابع مشابه
Bioremediation of lignocellulosic wastes of food industries by Aspergillus flavus as food and feed additive protein by solid-state fermentation process
The lignocellulosic wastes produced in food industries are suitable raw materials for the production of biological products. In this study, the solid state fermentation of Aspergillus flavus on lignocellulosic wastes was evaluated for microbial protein production. The fraction of the full factorial method was applied for experiment design and process optimization. The results analysis was perfo...
متن کاملAmylase production from Aspergillus oryzae LS1 by solid-state fermentation and its use for the hydrolysis of wheat flour
Nine Aspergillus and three of Trichoderma strains were grown on wheat bran (WB) medium under solid state fermentation (SSF) for amylase production. Aspergillus oryzae LS1 produced the highest level of the enzyme. The thermal stability profile of its crude enzyme revealed the half-life time of more than 2 h at 50 and 60ºC. The enzyme production was affected by strain type, incubation periods, le...
متن کاملXylanase Production under Solid State Fermentation by Aspergillus niger
Central composite orthogonal design was applied to quantify relations of xylanase production, loss of dry matter and change of pH with four critical variables during solid state fermentation of a mixture of wheat bran and wheat straw on which Aspergillus niger CCUG 33991 was cultivated. The studied variables included the percentage of wheat straw, temperature, moisture content, and fermentati...
متن کاملLipase Production in Solid State Fermentation Using Aspergillus niger: Response Surface Methodology
Among enzymes, lipases have been widely investigated because of the numerous industrial applications. In this study, optimization of lipase production by Aspergillus niger in solid state fermentation from rice bran as solid substrate was investigated. The optimal conditions with the aid of central composite design (CCD) under response surface methodology (RSM) were obtained. In the analysis of...
متن کاملProduction of Alkaline Protease by a New Aspergillus flavus Isolate under Solid-Substrate Fermentation Conditions for Use as a Depilation Agent.
The production of alkaline protease by an Aspergillus flavus strain isolated in our laboratory by solid-substrate fermentation for use as a depilation agent and the influence of various factors on enzyme production are reported. The optimum conditions for maximum production were a growth temperature of 32 degrees C, 63% substrate moisture, and a growth period of 48 h. Enrichment with corn steep...
متن کامل