The Incorporation of L-Asparagine-14C by Lymphoma 6C3HED Cells: Its Inhibition by Guinea Pig Serum1
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چکیده
was obtained with normal horse serum, which does not have antilymphoma effects and is known to be devoid of asparaginase. By contrast, under identical conditions in vivo, the 6C3HED cells failed to take up L-aspartic acid-14C,or did so only poorly. In vitro, the Lymphoma 6C3HED-OG cells also took up Lasparagine-14C avidly. Again, the incorporation of L-asparagineJ4C was almost completely prevented by guinea pig serum, either unheated or heated at 56°Cfor 30 min, whereas horse
منابع مشابه
The incorporation of l-asparagine-14C by lymphoma 6C3HED cells: its inhibition by guinea pig serum.
was obtained with normal horse serum, which does not have antilymphoma effects and is known to be devoid of asparaginase. By contrast, under identical conditions in vivo, the 6C3HED cells failed to take up L-aspartic acid-14C,or did so only poorly. In vitro, the Lymphoma 6C3HED-OG cells also took up Lasparagine-14C avidly. Again, the incorporation of L-asparagineJ4C was almost completely preven...
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Lymphoma 6C3HED-OG cells, known from previous work to be susceptible to the effects of guinea pig serum in vivo and dependent upon extrinsic asparagine for protein synthesis and growth in vitro, remained for the most part morphologically intact and countable in the electronic cell counter following exposures of 1 and 2 hr to the effects of heated (56 degrees C, 30 min) guinea pig serum injected...
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The antitumor activity of guinea 1'ig serum fractions was studied in vivo and in vitro by bright field, fluorescence, and electron microscopy observations and by dye permeability and cytidine-3H incorporation in 6C3HED lymphosarcoma cells. In C3H mice bearing the ascites tumor treated with the Lasparaginase fraction, there was a decrease in the total num ber and mitosis of tumor cells, a reduct...
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The antitumor activity of guinea 1'ig serum fractions was studied in vivo and in vitro by bright field, fluorescence, and electron microscopy observations and by dye permeability and cytidine-3H incorporation in 6C3HED lymphosarcoma cells. In C3H mice bearing the ascites tumor treated with the Lasparaginase fraction, there was a decrease in the total num ber and mitosis of tumor cells, a reduct...
متن کاملThe dependence of DNA and RNA synthesis on protein synthesis in asparaginase-treated lymphoma cells.
SUMMARY We studied the effects of asparagine depletion on macro molecular synthesis in an asparagine-dependent line of the 6C3HED lymphoma maintained in suspension culture. Aspar agine was removed from the culture by the asparaginase present in guinea pig serum or by deleting it in formulating the medium. Protein, RNA, and DNA synthesis were measured by the incorporation of labeled precursors a...
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