Morphology and Growth, Tumorigenicity, and Cytogenetics of Human Neuroblastoma Cells in Continuous Culture1
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چکیده
Continuous cell lines, SK-N-SH and SK-N-MC, were established in cell culture from human metastatic neuro blastoma tissue and maintained in vitro for 1 to 2 years. SK-N-SH comprises two morphologically distinctive cell types, a small spiny cell and a large epithelioid cell. SKN-MC is composed of small fibroblast-like cells with scant cytoplasm. In monolayer culture both cell lines form dis oriented growth patterns and reach high saturation den sities. Population-doubling times were 44 and 32 hr for SK-N-SH and SK-N-MC, respectively. Inoculum levels of IO7cells of both lines produced tumors confirmed by histo-
منابع مشابه
Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture.
Continuous cell lines, SK-N-SH and SK-N-MC, were established in cell culture from human metastatic neuro blastoma tissue and maintained in vitro for 1 to 2 years. SK-N-SH comprises two morphologically distinctive cell types, a small spiny cell and a large epithelioid cell. SKN-MC is composed of small fibroblast-like cells with scant cytoplasm. In monolayer culture both cell lines form dis orien...
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