The Chemistry of the Lipoids of Tubercle Bacilli
نویسنده
چکیده
The crude wax obtained from the chloroform extract of the human type of tubercle bacillus, Strain H-37, yielded on purification, as described in a former paper (l), two fractions; viz., a white solid substance designated as purified wax and a soft yellowish salve-like mass which was called soft wax. The present report deals with the analysis of the latter fraction. The work is far from complete and several constituents have not been identified. Our attention has been chiefly focused upon the nature of the fatty acids that were liberated on saponification, but incidentally some facts regarding other constituents have been observed and recorded. The water-soluble constituents, after saponification, contained only traces of reducing sugars and only a small amount of a syrupy substance was isolated, which probably consisted of glycerol since it gave a distinct acrolein reaction when heated with acid potassium sulfake. The ether-soluble components were found to consist of a small amount of a neutral perfume-like substance, a small quantity of unsaponifiable wax similar to that obtained from the purified wax (I), and a large percentage of true fatty acids. The fatty acids were separated by means of the lead soapether method according to the Gusserow-Varrentrapp (2, 3) pro-
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