Studies on proventriculitis in Broilers with molecular characterization to its viral causes

Infectious proventriculitis syndrome is at times a serious and ongoing problem causing significant financial losses. From the obtained results in the present study, it could be concluded that proventriculitis can be transmitted by oral inoculation of homogenates produced from proventriculi of birds with proventriculitis. Experimentally inoculated broilers were seroconverted to ARV, IBV and IBDV. I-FAT against ARV, IBV and IBDV detected intracytoplasmic staining in the proventricular glandular epithelial cells of experimentally inoculated broilers. ARV, IBV and IBDV were identified in proventricular homogenate inoculated broilers by RT-PCR. S1 sequence analysis of examined ARV isolates revealed a higher degree of similarity with published Egyptian isolates than that obtained when compared with international strains. S1 sequence analysis of examined IBV isolates revealed their close relatedness to Mass serotype. Nested PCR followed by RFLP and sequence analysis of HVR of VP2 gene revealed that the examined IBDV isolates related to vvIBDV. [Journal of American Science 2010; 6(9):582-592]. (ISSN: 15451003).