NMR assignment of the apo-form of a Desulfovibrio gigas protein containing a novel Mo-Cu cluster.

نویسندگان

  • Sofia R Pauleta
  • Américo G Duarte
  • Marta S Carepo
  • Alice S Pereira
  • Pedro Tavares
  • Isabel Moura
  • José J G Moura
چکیده

We report the 98% assignment of the apo-form of an orange protein, containing a novel Mo-Cu cluster isolated from Desulfovibrio gigas. This protein presents a region where backbone amide protons exchange fast with bulk solvent becoming undetectable. These residues were assigned using 13C-detection experiments.

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منابع مشابه

Crystallization and crystallographic analysis of the apo form of the orange protein (ORP) from Desulfovibrio gigas. Corrigendum

The orange-coloured protein (ORP) from Desulfovibrio gigas is a 12 kDa protein that contains a novel mixed-metal sulfide cluster of the type [S(2)MoS(2)CuS(2)MoS(2)]. Diffracting crystals of the apo form of ORP have been obtained. Data have been collected for the apo form of ORP to 2.25 A resolution in-house and to beyond 2.0 A resolution at ESRF, Grenoble. The crystals belonged to a trigonal s...

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Great Britain Oxidation - Reduction Studies of the Mo - ( 2 Fe - 2 S ) Protein from Desulfovibrio gigas 419 By JOSt J . G . MOURA and ANTONIO

Potentiometric titration followed by e.p.r. measurements were used to determine the midpoint reduction potentials of the redox centres of a molybdenum-containing ironsulphur protein previously isolated from Desulfovibrio gigas, a sulphate-reducing bacterium [Moura, Xavier, Bruschi, Le Gall, Hall & Cammack (1976) Biochem. Biophys. Res. Commun. 72, 782-789; Moura, Xavier, Bruschi, Le Gall & Cabra...

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Structural stability of adenylate kinase from the sulfate-reducing bacteria Desulfovibrio gigas.

A novel adenylate kinase (AK) has recently been purified from Desulfovibrio gigas and characterized as a Co(2+)/Zn(2+)-containing enzyme: this is an unusual characteristic for AKs from Gram-negative bacteria, in which these enzymes are normally devoid of metals. Here, we studied the conformational stability of holo- and apo-AK as a function of temperature by differential scanning calorimetry (D...

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عنوان ژورنال:
  • Biomolecular NMR assignments

دوره 1 1  شماره 

صفحات  -

تاریخ انتشار 2007